| Synonyms | Loading Control, Actin cytoplasmic 1, Beta-Actin, Review 008, FocusOn117 |
| Swiss Prot Num | P60709 |
| Immunogen | ACTB recombinant protein is used to produce this monoclonal antibody. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007) 2. Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007) 3. Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007) |
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| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| ? | Buffer System: PBS containing 0.09% (W/V) Sodium Azide as preservative. |
|
| Specificity | This antibody is specific to Beta-Actin. |
| Application | ELISA. Western blotting: 1/2000-1/10000. |
| Background | Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. Beta-actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |
| Concentration | 0.25 mg/ml |
| Review | r008 Antibody Panel to Actin |
| Focuson | 117 Antibodies to Loading Controls |
