| Immunogen | KLH conjugated synthetic peptide encoding 3xBtag (CQYPALTQYPALTQYPALT) was used as antigen. |
| Product Type | Antibodies |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| References | 1. Wang LF, Yu M, White JR, Eaton BT. BTag: a novel six-residue epitope tag for surveillance and purification of recombinant proteins. Gene. 1996 Feb 22;169(1):53-8. 2. du Plessis DH, Wang LF, Jordaan FA, Eaton BT. Fine mapping of a continuous epitope on VP7 of bluetongue virus using overlapping synthetic peptides and a random epitope library. Virology. 1994 Jan;198(1):346-9. |
|
| Format | State: Liquid purified Ig fraction. |
| ? | Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| ? | Buffer System: PBS containing 0.09% (W/V) Sodium Azide as preservative. |
|
| Specificity | B Tag specific. |
| Application | ELISA: 1/5,000. Western Blot. |
| Background | Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. BTag is an epitope tag composed of a 6 residue peptide, QYPALT, derived from the highly conserved region of the major core protein, VP7, of bluetongue (BT) viruses. This polyclonal antibody provides a simple solution to detect the expression of a B-tagged protein in cells. |
| Caution | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Concentration | 0.25 mg/ml |
