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Description |
Unit |
Price
(VAT별도) |
납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-345343 |
BM5015 |
Adeno-Associated Virus (AAV) - VP1/VP2/VP3//Human, Monkey/Mouse |
1/EA |
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Subject
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Adeno-Associated Virus (AAV) - VP1/VP2/VP3 |
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Description
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/ Human, Monkey/Mouse |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Frozen sections, Immunofluorescence, Western blot / Immunoblot, Immunoprecipitation |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 50 ug | | Reactivity | Human, Monkey | | Presentation | Purified | | Applications | Frozen sections, Immunofluorescence, Western blot / Immunoblot, Immunoprecipitation | | Isotype | IgG1 | | Host | Mouse | | Clone | B1 | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | AAV2 | | Immunogen | Adeno-associated virus capsid proteins and virus particles. | | Product Type | Antibodies | | Recommended Isotype Control | SM10P (for use in human samples), AM03095PU-N | | Storage | Store the antibody at 2-8°C after reconstitution. Shelf life: One year from despatch. | | References | 1. Wistuba A.,Weger S., Kern A., and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly: Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319. 2. Wistuba A., Kern A., Weger S., Grimm D., and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352. 3. Wobus C.A., Hügle-Dörr B., Girod A., Petersen G., Hallek M. and Kleinschmidt J.A. (2000) Monoclonal antibodies against the adenoassociated virus type 2 (AAV-2) capsid: Epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J. Virology 74, 9281-9293. | |
| Format | Reconstitution: Reconstitute with 1 ml distilled water | | ? | State: Lyophilized purified IgG fraction with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer. | | ? | Purification: Affinity Chromatography on Protein A . | |
| Specificity | Useful for Immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. BM5010). B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. Epitope mapping experiments (Wobus et al., see below) identified aa726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region. The antibody is also useful for characterization of different stages of infection. Reacts with AAV-2, found in human and monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction). | | Application | Immunofluorescence Microscopy. Immunoprecipitation (mainly free VP proteins). Immunohistochemistry on Frozen Sections (Fixation: Acetone or formaldehyde). Working Dilution: 1/10 with PBS or Tris-buffered saline. Incubation Time: 1h at RT. Immunoblotting (Western Blot). Affinity Chromatography. | | Species Reactivity | Tested: Human and Monkey. | |
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