| Catalog Number | GTX21187 |
| Product Name | 6X His tag? antibody (HRP) |
| Product Description | Rabbit polyclonal to 6X His tag (HRP) |
| Synonyms | 6X His epitope tag HHHHHH epitope tag HHHHHH tag |
| Background | This antibody recognises proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Isotype | IgG |
| Immunogen | Synthetic peptide: 6-His (HHHHHH) conjugated to KLH. |
| Antigen Species | N/A |
| Tested Applications | ELISA, Immunocytochemistry, Immunohistochemistry, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For ELISA: Use at a concentration of 0.01-1 μg/ml. For ICC: Use at a concentration of 0.5-2 μg/ml. For IHC: Use at a concentration of 0.5-2 μg/ml. For WB (Colorimetric): Use at a concentration of 0.1-1 μg/ml. For WB (Chemiluminescent): Use at a concentration of 0.03-1 μg/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Target | 6X His tag? |
| Conjugation | HRP |
| Conjuation Note | Conjugated to horseradish peroxidase (HRP) |
| Form Supplied | Liquid |
| Purification | Immunogen affinity purified |
| Storage Buffer | 50mM HEPES, pH 7.1, 0.1M NaCl containing 0.2% BSA and 0.1% Pro-Clean 400 |
| Storage Instruction | Keep as concentrated solution, aliquot and store at 4°C. |
