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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-24-356913 |
SP6372P |
I Kappa B alpha (IKBa)//Human, Mouse, Rat/Rabbit |
1/EA |
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Subject
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I Kappa B alpha (IKBa) |
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Description
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/ Human, Mouse, Rat/Rabbit |
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Clonality
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Mono |
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Company
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Acris Antibodies GmbH |
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Application
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Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation |
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Conjugation
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Immunogen
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Contents
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Description: | Manufacturer | Acris Antibodies GmbH | | Quantity | 50 ug | | Reactivity | Human, Mouse, Rat | | Presentation | Aff - Purified | | Applications | Enzyme Immunoassay, Western blot / Immunoblot, Immunoprecipitation | | Host | Rabbit | | PDF datasheet | view | | Shipping to | Worldwide | | Synonyms | NFKBIA, IKBA, MAD3, NFKBI, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha | | Swiss Prot Num | P25963 | | Immunogen | Synthetic peptide corresponding to human I?ºB?± around serines 32 and 36 | | Product Type | Antibodies | | Storage | Store the antibody at 2 - 8 °C or (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. | | References | 1. Karin, M et al (2000) Annu. Rev. Immunol. 18: 621-663. 2. Karin, M. et al. (2001) Nat. Immunol. 3: 221-227. | |
| Format | State: Liquid Ig fraction | | ? | Purification: Epitope affinity chromatography | | ? | Buffer System: 1x PBS (pH 7.4) containing 0.05 % sodium azide | |
| Specificity | This antibody is specific for human I?ºB?±. It was evaluated by ELISA, dot blot and Western blot. It only recognized the human I?ºB, not other phosphorylated or non-related peptides. By immunoblot assay, an immunoreactive band at ~40 kDa from Hela, 3T3, THP-1 whole cell lysate was detected. This immunoreactive band is abolished by the depletion of the immunizing peptide. | | Application | ELISA: 0.1-1.0 ?g/ml. Western blot: 0.5-2.0 ?g/ml. Immunoprecipitaion (recommended): 3.0-5.0 ?g/extract from 10e7 cells. | | Background | NF-?ºB is a family of transcription factors, including p50, p52, p65, RelB, and C-Rel (1). It regulates the expression of a number of proteins involved in the regulation of cell survival and immune responses (2). The biological activity of NF-?ºB is tightly controlled by its inhibitor protein I?ºB, which binds to and sequesters NF-?ºB in the cytoplasm. There are three isoforms of the I?ºB: I?ºB?±, -?² and -u. I?ºB?±, the well-characterized member of the I?ºB family, contains both nuclear localization sequence and nuclear export signal that render to I?ºB?± the activity to rapidly shuttle between the nucleus and the cytoplasm. The I?ºB?± protein binds to NF-?ºB dimmers, masking their nuclear localization sequences and causing their cytoplasmic retention. After NF-?ºB positive stimuli, I?ºB kinase complex phosphorylates I?ºBs in two conserved serines (Ser-32 and Ser-36 in I?ºB?±), leading to ubiquitination and subsequent proteasomal degradation (1). | | Concentration | 0.2 mg/ml | |
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