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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-310227 |
NB100-92140 |
A RAF Antibody//Human, Mouse,/Rabbit |
1/EA |
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문의 |
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Subject
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A RAF Antibody |
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Description
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/ Human, Mouse,/Rabbit |
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Clonality
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Poly |
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Company
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Novus biologicals |
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Application
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ELISA, IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Rt, Mu, Hu | | Applications: | IHC, WB, ELISA | | Clonality: | Polyclonal | | | Gene: | ARAF | | Purity: | Immunogen affinity purified | | Host: | Rabbit | | | Specificity: | A-RAF (D297) detects endogenous levels of A-RAF protein. | Details: Gene Symbol: ARAF | Entrez: | 369 (Human) | | | 64363 (Rat) | | Related Genes: | RAF1, ZHX2, BRAF, NDUFAB1, SNRPE, FASN, OXSM, MAPK1, EPHB2, FAS, MAP2K1, PIK3CG, LYST, MAPK3, TIMP1, SHBG, ABP1, MCAT, EGF, GALP, AKT1, SRC, GLYAT, SYN1, CAT, TH1L, C3orf39, CFP, CYP19A1, PKC, MAP3K1, FOS, PIK3CA, PIK3CB, PIK3CD, A4GALT, HSP90AA1, RRAS2, KCNH8, CDKN1A | Background: Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue. Species: | Reactivity: | This antibody is reactive against Human, Mouse, Rat. | Applications: | Uses: | Western blot analysis of A-RAF (D297) antibody in extracts from HeLa cell treated with PMA 125ng/ml 30' Immunohistochemistry analysis of A-RAF (D297) antibody in paraffin-embedded human breast carcinoma tissue. | | Dilutions: | ELISA 1:10000-1:20000, Western Blot 1:500-1:1000, immunohistochemistry 1:50-1:200, | | Concentrations: | 1.0 mg/ml | Packaging: | Buffer: | Phosphate buffered saline, pH 7.2. | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 15mM Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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