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Description |
Unit |
Price
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납기 |
재고 |
DataSheet |
주문/견적 |
| LCA-23-313920 |
NB600-1079 |
ABCG2 Antibody (BXP-9)//Mouse/Rat |
1/EA |
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문의 |
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Subject
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ABCG2 Antibody (BXP-9) |
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Description
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/ Mouse/Rat |
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Clonality
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Mono |
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Company
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Novus biologicals |
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Application
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FACS, ICC, IHC-P, Western Blot |
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Conjugation
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Immunogen
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Contents
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Description:Summary: | Species: | Mu | | Applications: | ICC, WB, IHC, FACS | | Clonality: | Monoclonal | | | Gene: | ABCG2 | | Purity: | Tissue culture supernatant | | Host: | Rat | | | Specificity: | The BXP-9 Mab was selected after immunization with a fusion protein containing the E. coli maltose binding protein and a fragment of the mouse bcrp protein corresponding to amino acids 221-394. BXP-9 reacts with an internal epitope of bcrp, a 70 kD transmembrane half-transporter which is involved in Multidrug resistance. BXP-9 does not react with the human BCRP molecule. | Details: | Clone: | BXP-9 | | Isotype: | IgG1 | | Immunogen: | Synthetic peptide, corresponding to amino acids 221-394 of mouse BCRP/ABCG2 fused to maltose binding protein. | | Localization: | Plasma membrane, integral membrane protein | Gene Symbol: ABCG2 | | 26357 (Mouse) | | Related Genes: | ABCB1, ABCC1, ABCC2, MSH3, MDM4, TBC1D9, KLHL1, MARCKSL1, GPR177, MVP, BANF1, PGPEP1, ABCC3, ABCC4, EGFR, ABCC5, LRP1, PTPRA, RPSA, SLCO1B1, ABCB4, MTX1, ABCB11, CD34, CD9, CYP3A4, SLCO1A2, CKAP4, NR1I2, TP63, SLCO1B3, SLCO2B1, NES, GSTP1, UVRAG, RPE65, SLC22A1, TOP1, CFTR, KRT3 | Background: An ABC transporter. Allows efflux of Hoechst dye, a property that has been used to separate bone marrow side population cells, which express BCRP/ABCG2. Appears to play a major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cell line. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. Species: | Reactivity: | This antibody reacts with mouse. It does not react with human. Not yet tested in other species. | Applications: | Uses: | Immunocytochemistry: working dilution of 1:20 - 1:50 on acetone fixed cytospin preparations. Immunohistochemistry: BXP-9 (dilution of 1:20) on acetone fixed frozen sections can be followed by incubation with biotin-labeled rabbit anti-rat IgG (1:100, DAKO) and HRP-labeled streptavidin (1: 500, DAKO). Western Blotting: working dilution of 1:20 - 1:50 and HRP-anti-rat-IgG. Flow Cytometry: optimal conditions still to be defined. | | Dilutions: | flow cytometry / FACS analysis , immunohistochemistry , Western Blot , Immunocytochemistry 1:20 | | Concentrations: | 0.25 mg/ml | Packaging: | Buffer: | 0.7% BSA, tissue culture supernatant (serum free), 0.1% Sodium Azide | | Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. | | Preservative: | 0.1% Sodium Azide | | Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. | |
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